Vaccines and Immunization

Biography

Biography: Yoshinori Hayakawa

Abstract

New influenza pandemic might kill 300 million people in the world. Ordinary vaccines are too costly for many people in developing countries. Moreover vaccines are not available in early stages of pandemic. Ferret nasal mucosa is carcinized using carcinogen for easiness of incubation. Bird influenza virus is attenuated by reverse genetics. The virus is marked by green fluorescent protein. This attenuated virus is sprayed to many cultured cancer cell specimen incubated. In some specimen attenuated virus will mutated to increase in cancer cells, checked by green fluorescence. Then the virus is tested to infect ferret and then human volunteers without serious symptom. Virus with strongest virus titer to infect ferret is selected as seed virus of infectious attenuated live vaccine. The seed virus will be increased in incubated cancer cells by bioreactors all over the world and sprayed to vulnerable people, e.g. soldiers, students, people in slams, medical staffs and people engaged in lifeline. Drones may be used to enhance infection, spraying in slams and markets thin capsules including the live vaccine. Thin capsules are melted at human nasal mucous membrane. Thus basic immunity is gained by many people against dangerous virus. Artificial pandemic of dangerous virus as H7N9, H5N1 etc. are to be created serially with few years interval. Artificial pandemic should be initiated before wild type pandemic starts. One reason is to avoid reassortment (mixture) of virus RNA and another is to avoid clinical and social confusion. It should not overlap with influenza season. The number of victims seems to be less than 1000^th of wild type pandemic. The calculation assumes the number of victims with infectious attenuated live vaccine is less than that of A/H1N1. Similar technique cannot be used for creating biological weapon as toxic virus kill cancer cells for incubation.